![]() I used a software program to capture images from my cameras called Digicam control which is free. It is important that adjacent images overlap on all four sides by 25-30%. ![]() Smaller pans of 20-30 images can be made by moving the slide by hand, but it is easier with a mechanical stage. To make large panoramas with a light microscope a mechanical stage with XY translation is necessary to move the microscope slide. Eventually, I was able to produce a large image 40 feet tall at 300 dpi (dots per inch) using a combination of Adobe Photoshop CS2023 and Topaz Gigapixel. I also found one free software program that could handle large numbers of images and also produce panoramic images from a video taken of the specimen ( Microsoft ICE – image composite editor). An automatic scanning system is more costly, but it saves a tremendous amount of time when compared to stitching images manually. The use of digital images taken with a microscope is referred to as virtual microscopy. I also show one system by Motic that automatically stitches images from prepared specimens on microscope slides. Here I describe methods I used to make a large panorama of a leaf and some of the challenges I had to overcome in doing so. I have also taken thousands of photomicrographs and with several kinds of light microscopes and all of my microscopes had attached high resolution Nikon or Canon digital single lens reflex cameras (DSLRs). But these photos were all landscape panoramic images or pictures of the night sky and aurora. I have made many large panoramas before some of them 10 feet tall and 30 feet wide with no problem using Photoshop. ![]() This seemed like it should be straight forward, but it wasn’t.Īspen leaves were collected in September around Calgary with the help of my father and wife. Many images would need to be taken of the leaf and then stitched together to form a giant panorama. The image was to be a photographed with a light microscope at 200X so that the individual cells and organelles with the leaf could be seen when standing close to the enlarged image. In 2022, I was contacted and asked if I could produce a picture of an aspen leaf enlarged to 40 feet tall. View interactive zoomable image on Motic's server. Light-Microscope of Aspen and Elodea LeavesĪspen leaf panorama stitched from several hundred images, each image photographed with a 20X objective.
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